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Suggested Values and Protocols for Use of Extended Porcine Semen in an Artificial Insemination (AI) Program

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Semen Variable	Value
Method of insemination	Cervical (CAI) or intrauterine (IUI) AI
Number of inseminations	2
Sperm/dose	1.5 (IUI) or 2.5–3.0 × 10⁹ (CAI) sperm
Gross sperm motility	≥70%
Normal sperm morphology	≥75%
Sperm concentration	25 to 65 × 10⁶ sperm/mL
Dose volume (cervical deposition)	45 mL (IUI) or 70–80 mL (CAI)
Microbial content	No appreciable aerobic growth
Arrival temperature	15º–19ºC